MUC13 Cell Surface Mucin Limits Salmonella Typhimurium Infection by Protecting the Mucosal Epithelial Barrier

Michael A McGuckin; Julie M Davies; Pascal Felgner; Kuan Yau Wong; Rabina Giri; Yaowu He; Md Moniruzzaman; Thomas Kryza; Haressh Sajiir; John D Hooper; Timothy H Florin; Jakob Begun; Abderrahim Oussalah; Sumaira Z Hasnain; Michael Hensel; Yong H Sheng; Michael A McGuckin

Journal article

MUC13 Cell Surface Mucin Limits Salmonella Typhimurium Infection by Protecting the Mucosal Epithelial Barrier

Michael A McGuckin, Julie M Davies, Pascal Felgner, Kuan Yau Wong, Rabina Giri, Yaowu He, Md Moniruzzaman, Thomas Kryza, Haressh Sajiir, John D Hooper, Timothy H Florin, Jakob Begun, Abderrahim Oussalah, Sumaira Z Hasnain, Michael Hensel, Yong H Sheng, Michael A McGuckin

Cellular and Molecular Gastroenterology and Hepatology | Elsevier | Published : 2023

DOI: 10.1016/j.jcmgh.2023.08.011

Abstract

BACKGROUND & AIMS: MUC13 cell surface mucin is highly expressed on the mucosal surface throughout the intestine, yet its role against bacterial infection is unknown. We investigated how MUC13 impacts Salmonella typhimurium (S Tm) infection and elucidated its mechanisms of action. METHODS: Muc13-/- and wild-type littermate mice were gavaged with 2 isogenic strains of S Tm after pre-conditioning with streptomycin. We assessed clinical parameters, cecal histology, local and systemic bacterial load, and proinflammatory cytokines after infection. Cecal enteroids and epithelial cell lines were used to evaluate the mechanism of MUC13 activity after infection. The interaction between bacterial SiiE ..

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